Analysis of the genotoxic effects of mobile phone radiation. Changes in buccal cytome biomarkers in relation to ageing and. A buccal cytome assay was used to measure ratios of buccal cell populations and micronuclei in clinically diagnosed alzheimers patients compared to age and gendermatched controls. Pdf the buccal micronucleus cytome bmcyt assay is a new minimally invasive system for studying dna damage, chromosomal instability, cell death, and. Buccal micronucleus cytome assay bmn measures changes in differentiation as the frequency of basaldifferentiated, binuclear, and cells in different phases of cell deathapoptosisnecrosis cells with condensed chromatin, karriorhectic. Methodology for detection of mn and other nuclear anomalies in exfoliated buccal epithelial cells. Automation of the buccal micronucleus cytome assay using. Buccal micronucleus cytome assay springer nature experiments. The micronucleus assay in toxicology is the first comprehensive volume concerning the use of micronucleus assays in genetic toxicology. Pdf buccal micronucleus cytome assay stefano bonassi. N2 the micronucleus mn assay, applied in different surrogate tissues, is one of the best validated cytogenetic techniques for evaluating chromosomal damage in humans. Bolognesi c, knasmueller s, nersesyan a, thomas p, fenech m. The aim of this study was to assess dna damage in turkish coal miners with the buccal micronucleus cytome bmcyt assay as the least invasive and therefore most practical method that may find wider application in coal miner biomonitoring. The humn xl project evaluates the impact of host factors.
Arul p, shetty s, masilamani s, akshatha c, naveen kumar b j. Perspectives revisitedthe buccal cytome assay in mobile phone. The human micronucleus project on exfoliated buccal cells. Micronuclei in exfoliated buccal cells of children living in a cluster. Alzheimers disease ad is a progressive degenerative disorder of the brain and is the commonest form of dementia. Pdf application of the buccal micronucleus cytome assay. The micronucleus assay in toxicology rsc publishing. Overall, the mean mn frequency decreased significantly from winter to latespring. Research article open access the effects of sex, age and. Mar 25, 2016 the human micronucleus project on exfoliated buccal cells humnxl.
Comprehensive and current, dna damage detection in situ, ex vivo, and in vivo. The mn test is used as an indicator of genotoxic exposition, since it is associated with chromosome aberrations. Micronucleus assay in exfoliated buccal epithelial cells. The objective of this study was to assess the incidence of micronuclei mn in buccal mucosal cells of dental technicians exposed to mma using buccal micronucleus cytome bmcyt assay. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the humn xl collaborative study. Tobacco farmers apply large amounts of pesticides to control insect growth. With normal ageing a significant increase in cells with micronuclei p buccal micronucleus cytome bmcyt assay is a new minimally invasive system for studying dna damage, chromosomal instability, cell death, and the regenerative potential of buccal mucosal tissue.
Our results showed the baseline buccal micronucleus frequency was 0. Buccal micronucleus cytome assay in primary school children. Micronucleus cytome assays in human lymphocytes and buccal. Buccal micronucleus cytome assay bmcyt abstract the present study is the. This method is increasingly used in molecular epidemiological studies for investigating the impact of nutrition, lifestyle factors, genotoxin. The buccal micronucleus cytome assay was applied in the mapec study. Nov 01, 2011 the human buccal micronucleus cytome assay bmcyt is one of the most widely used techniques to measure genetic damage in human population studies. The various cell types and nuclear anomalies among the various cell types in the buccal mucosa, which are observed and scored in a buccal micronucleus cytome assay, are shown schematically in fig. The buccal micronucleus cytome bmcyt assay is a minimally invasive approach for measuring dna damage, cell proliferation, cell differentiation and cell death in exfoliated buccal cells. Jun 30, 2014 the buccal micronucleus cytome assay is a noninvasive method used to study dna damage, chromosomal instability, cell death, and the regenerative potential of human buccal mucosal tissue. The humnxl scoring criteria for different cell types and nuclear anomalies in the buccal micronucleus cytome assay an update and expanded photogallery. Concerns about the adverse health effects of chemicals and radiation present in the environment and at workplaces have created the need for better detection systems to assess their potential to cause dna damage in humans and other organisms across ecosystems.
The buccal micronucleus cytome assay was used to measure ratios of buccal cell populations and micronuclei in clinically diagnosed alzheimers patients compared to age and gender matched controls. Frequencies of basal cells p buccal micronucleus cytome bmcyt assay has been used widely to investigate biomarkers for dna damage, cell death, and basal cell frequency in buccal cells. Assessment of genetic damage in buccal epithelium cells of. It was a crosssectional observational study and was carried on saliva samples of sca patients taken from thalassemia and sickle cell society, hyderabad, india. Request pdf on jan 1, 2015, abhay singh yadav and others published buccal micronucleus cytome assay a biomarker of genotoxicity find, read and cite all the research you need on researchgate. N2 the micronucleus mn assay, applied in different surrogate tissues, is one of the best validated cytogenetic techniques for evaluating chromosomal damage in. Other nuclear anomalies in buccal cells were measured through the use of the buccal micronucleus cytome assay. Workers exposed to wood dust have an increased micronucleus.
The buccal micronucleus cytome bmcyt assay, based on scoring not only micronucleus frequency but also other genome damage markers, dead or degenerated cells, provides a measure of cytotoxic and genotoxic effects. The buccal micronucleus cytome assay is a noninvasive method used to study dna damage, chromosomal instability, cell death, and the regenerative potential of human buccal mucosal tissue. Assessment of listerine cool mint mouthwash influence on. Methods and protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology. The buccal micronucleus cytome bmcyt assay is a minimally invasive method for studying dna damage, chromosomal instability, cell death and the regenerative potential. Overall, the mean mn frequency decreased significantly from winter to. The micronucleus mn assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans exposed to occupational and environmental agents. Buccal micronucleus cytome assay in sickle cell disease. The aim of this study was to assess whether prolonged exposure to coal dust could lead to an increase in genomic instability, cell death, and frequency of basal cells using the. Dna damage assessment with buccal micronucleus cytome assay.
The main limitation for its use is the lack of knowledge about inter and intralaboratory variability in scoring micronuclei and other end points included in. The buccal micronucleus cytome bmcyt assay is a minimally invasive. Significant correlations amongst cell death biomarkers observed in this study were used to propose a new model of the interrelationship of cell types scored within the buccal micronucleus cytome assay. To our knowledge, no studies have as yet assessed buccal cell micronuclei in patients with connective tissue diseases or inflammatory arthritis. The micronucleus cytome assay applied in buccal exfoliated cells bmncyt, provides a complementary method for measuring dna damage and cytotoxic effects in an easily accessible tissue not requiring ex vivoin vitro culture. Evaluation of methyl methacrylate monomer cytotoxicity in. Methyl methacrylate mma monomer, a primary component of dental resins, is known to induce cytotoxicity, dermatitis, and neuropathy.
The buccal micronucleus cytome bmcyt assay is a minimally invasive method for studying dna damage, chromosomal instability, cell death. The buccal micronucleus cytome bmcyt assay is a new minimally invasive system for studying dna damage, chromosomal instability, cell death, and the regenerative potential of buccal mucosal tissue. Mn count in the exfoliated cells can be used as a marker for an abnormal cell cycle as it is formed as a result of aberrant mitosis when the whole chromosome or chromatid fragment fails to reach the spindle pole. The study aim was to assay the genomes stability in patients with sickle cell anaemia by a micronucleus assay in buccal mucosa cells. The various cell types scored in the buccal micronucleus cytome assay adapted from thomas et al. The micronucleus mn assay in human exfoliated cells. The casecontrol study was conducted at the lahore college for women university, lahore, pakistan, from september 2014 to february 2015, and comprised petrol pump workers. Buccal micronucleus cytome assay in primary school. Application of the buccal micronucleus cytome assay and analysis of pon1gln192arg and cyp2a69.
The bmcyt assay was performed according to the procedure described by thomas and fenech. The buccal micronucleus cytome assay was used to investigate biomarkers for dna damage, cell death and basal cell frequency in buccal cells of healthy young, healthy old and young downs syndrome cohorts. Buccal micronucleus cytome biomarkers may be associated. Frequencies of basal cells p buccal epithelial cells, liquidbased cytology, micronucleus, petrol station workers how to cite this article.
During the years 20142016 the university of salento performed the impact of air quality on health of residents in the municipalities of cutrofiano, galatina, sogliano cavour, soleto and sternatia imp. Mn frequencies in nasal and buccal cells were significantly higher in the exposed group compared to the nonexposed group. Dna damage detection in situ, ex vivo, and in vivo methods. This method is increasingly being used in molecular epidemiologic studies investigating the impact of nutrition, lifestyle factors, genotoxin.
Nowadays, the human buccal micronucleus cytome bmcyt assay is one of the most widely used techniques to measure genetic damage in studies on human population exposed to airborne pollutants 19,20. In this study micronucleus count in exfoliated buccal mucosal cells were used to evaluate the genotoxic effect of mobile phone radiation. Air study, an epidemiologicalmolecular research project aiming to evaluate early dna damage in children living in an area of salento with high incidence of lung cancer among the male. Micronucleus frequency in exfoliated buccal cells of children. Buccal micronucleus cytome assay italian ministry of health. The buccal micronucleus cytome assay in exfoliated buccal cells is utilized as biomarkers for dna damage, cell death and basal cell frequency. Buccal micronucleus cytome biomarkers may be associated with. The buccal micronucleus cytome bmcyt assay is a minimally invasive method for studying dna damage, chromosomal instability, cell death and the regenerative potential of human buccal mucosal tissue. The frequency of mn and metanuclear alterations was evaluated in 1046 children. Cytogenetic biomonitoring in fuel station attendants of. Application of the buccal micronucleus cytome assay and analysis of. Dna damage assessment with buccal micronucleus cytome assay in turkish coal miners.
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